Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Propagation Channel Characterization for 6–14 GHz Bands Based on Large Array Measurement for Indoor Scenarios

The demand for wideband data transmission in the “next-generation” mobile communication system is growing rapidly. As the frequency band around 10 GHz could be the option for 6th-generation (6G) wireless communication systems. In this paper, a recently conducted measurement campaign in the 6–14 GHz radio wave propagation channel using a large antenna array with 1024 elements is introduced. In order to investigate the behavior of the wideband channel, we have analyzed the channel characteristics with respect to different carrier frequencies, bandwidths, the locations of antenna elements, and user locations, aiming at exploring the spatio-frequency variability of channels in the massive multiple-input multiple-output (MIMO) scenarios. Moreover, the sparsity of the channel in frequency and spatial domains is evaluated through the degree of freedom (DoF) analysis, and statistical models are established

Propagation Channel Characterization for 6–14 GHz Bands Based on Large Array Measurement for Indoor Scenarios

The demand for wideband data transmission in the “next-generation” mobile communication system is growing rapidly. As the frequency band around 10 GHz could be the option for 6th-generation (6G) wireless communication systems. In this paper, a recently conducted measurement campaign in the 6–14 GHz radio wave propagation channel using a large antenna array with 1024 elements is introduced. In order to investigate the behavior of the wideband channel, we have analyzed the channel characteristics with respect to different carrier frequencies, bandwidths, the locations of antenna elements, and user locations, aiming at exploring the spatio-frequency variability of channels in the massive multiple-input multiple-output (MIMO) scenarios. Moreover, the sparsity of the channel in frequency and spatial domains is evaluated through the degree of freedom (DoF) analysis, and statistical models are established

ELAA Channel Characterization with Parameter Estimation Based on a Generalized Array Manifold Model

The extremely large antenna arrays (ELAAs) and millimeter-wave systems have become key techniques for obtaining higher frequency spectrum efficiency in sixth-generation (6G) communication systems. It is necessary to determine appropriate statistical models to describe the channel characteristics for the ELAAs, such as spatial non-stationarity, dispersion in angular domain, and spatial consistency. Thus, a signal model based on the generalized array manifold (GAM) that describes the dispersion in direction using the definition of the slightly distributed scatterer (SDS) is proposed in this work. An estimator for the parameters of the GAM model, namely GAM Space-Alternating Generalized Expectation-maximization (GAM-SAGE), is also designed. Moreover, a method to obtain a stochastic SDS-based channel model (SBCM) that is capable of reproducing the spatial consistency is proposed. The method is then used to establish measurement-based models for line-of-sight (LoS) and non-line-of-sight (NLoS) scenarios using a 40×40 receiver (Rx) planar antenna array at a carrier frequency of 40 GHz. The results demonstrate that the SBCM is capable of achieving spatially consistent results and outperforms the specular-path (SP) models in completely characterizing the ELAA channels at millimeter-wave bands, which are fundamental for the design of 6G

Data-driven modelling of product crystal size distribution and optimal input design for batch cooling crystallization processes

In this paper, a novel data-driven model building method is proposed for predicting one-dimensional product crystal size distribution (CSD) or chord length distribution (CLD) of batch cooling crystallization processes, based on only batch run data. The proposed model relating the manipulated variable of cooling rate to the product CSD are constructed by two classes of basis functions, one is the wavelet basis function for reshaping the CSD and the other is the polynomial basis function for weighting the chosen wavelet basis functions to reflect the nonlinear relationship between the input and the density of individual crystal size among the product crystals. Correspondingly, a double-layer least squares algorithm is established to estimate the model parameters, along with an adaptive strategy to determine the location and number of wavelet basis functions. By introducing an objective function that combines the information entropy of product CSD and the sample deviation of product crystals in each batch with respect to the target crystal size, the optimal input design of cooling rate for the desired product CSD is carried out by using a particle swarm optimization (PSO) algorithm to solve the non-convex optimization problem with the established CSD model. Simulation tests on the hen-egg-white lysozyme crystallization process along with experiments on the L-glutamic acid cooling crystallization process are performed to demonstrate the effectiveness and advantage of the proposed method

Recursive UE Localization for a Multi-RIS-Assisted Wireless System in an Obstacle-Dense Environment

Accurate user equipment (UE) localization in an obstacle-dense environment is quite challenging due to the insufficiency of line-of-sight (LoS) links. However, the reconfigurable intelligent surface (RIS) has the potential for offering alternative RIS-assisted LoS links to refine the localization results. In this paper, a recursive localization scheme is proposed based on an iterative RIS selection strategy, with the help of prior knowledge of the propagation environment. And numerical results based on a geometry-based channel simulator in a typical composite urban environment exhibit the improvement of localization accuracy

Unique microbial landscape in the human oropharynx during different types of acute respiratory tract infections

Abstract Background Secondary bacterial infections and pneumonia are major mortality causes of respiratory viruses, and the disruption of the upper respiratory tract (URT) microbiota is a crucial component of this process. However, whether this URT dysbiosis associates with the viral species (in other words, is viral type-specific) is unclear. Results Here, we recruited 735 outpatients with upper respiratory symptoms, identified the infectious virus types in 349 participants using multiplex RT-PCR, and profiled their upper respiratory microbiome using the 16S ribosomal RNA gene and metagenomic gene sequencing. Microbial and viral data were subsequently used as inputs for multivariate analysis aimed at revealing viral type-specific disruption of the upper respiratory microbiota. We found that the oropharyngeal microbiota shaped by influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), and human rhinovirus (HRV) infections exhibited three distinct patterns of dysbiosis, and Veillonella was identified as a prominent biomarker for any type of respiratory viral infections. Influenza virus infections are significantly correlated with increased oropharynx microbiota diversity and enrichment of functional metabolic pathways such as L-arginine biosynthesis and tetracycline resistance gene tetW. We used the GRiD algorithm and found the predicted growth rate of common respiratory pathogens was increased upon influenza virus infection, while commensal bacteria, such as Streptococcus infantis and Streptococcus mitis, may act as a colonization resistance to the overgrowth of these pathogens. Conclusions We found that respiratory viral infections are linked with viral type-specific disruption of the upper respiratory microbiota, particularly, influenza infections uniquely associated with increased microbial diversity and growth rates of specific pathogens in URT. These findings are essential for clarifying the differences and dynamics of respiratory microbiota in healthy participants and acute respiratory viral infections, which contribute to elucidating the pathogenesis of viral-host-bacterial interactions to provide insights into future studies on effective prevention and treatment of respiratory tract infections. Video Abstrac